Other

Part:BBa_I716309:Design

Designed by: Vincent Parker   Group: iGEM07_Berkeley_UC   (2007-10-25)


T7-RBS-OtsB-RBS-OtsA-TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2446
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Digest I716308 (Bsa1, BglII, 970, 3489, L) Sub into Bca1144-I716306 (Bsa1, Alwn1, BamHI 548, 1119, 549, L) Product I716309

Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References